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mouse anti dac  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti dac
    Mouse Anti Dac, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+dac/anti-Cadherin/bio_rxiv__64898__2026__04__06__716791-188-56-87
    Average 94 stars, based on 27 article reviews
    mouse anti dac - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: KDM5-mediated transcriptional activation of ribosomal protein genes alters translation efficiency to regulate mitochondrial metabolism in neurons.
    Article Snippet: .. A tet-inducible expression ystem was introduced, and lentivirus particles prepared rom the plasmid vectors; pLV_TRET_hNgn2_UBC_Puro RRID:A ddgene_61474) and FUdeltaGW -rtT A ( RRID: ddgene_19780), followed by treatment with doxycycline nd selection with puromycin. ntibodies he following primary antibodies were used: Anti-Fas2 1:25, Developmental Studies Hybridoma Bank (DSHB) at# 1D4 anti-Fasciclin II, RRID:AB _ 528235 ), Anti-HA 1:1000, Cell Signaling Technology cat# 3724, RRID: B _ 1549585 ), anti-H3 (1:20000, Active Motif Cat# 39763, RID:AB _ 2650522 ), anti-H3K4me3 (Cell Signaling Techology Cat# 9751, RRID:AB _ 2616028 ), Anti-FLAG (Sigmaldrich Cat# F1804, RRID:AB _ 262044 ), Anti-vGLUT2 (1:200; Millipore #mab5504) Anti-TUJ (1:500; Genescript #A01627), Anti-Cyclophilin B (Thermofisher #PA1-027A), anti-DAC (1:50; DSHB cat# mAbdac2-2; RRID:AB _ 528190 ). ..

    Plasmid Preparation:

    Article Title: KDM5-mediated transcriptional activation of ribosomal protein genes alters translation efficiency to regulate mitochondrial metabolism in neurons.
    Article Snippet: .. A tet-inducible expression ystem was introduced, and lentivirus particles prepared rom the plasmid vectors; pLV_TRET_hNgn2_UBC_Puro RRID:A ddgene_61474) and FUdeltaGW -rtT A ( RRID: ddgene_19780), followed by treatment with doxycycline nd selection with puromycin. ntibodies he following primary antibodies were used: Anti-Fas2 1:25, Developmental Studies Hybridoma Bank (DSHB) at# 1D4 anti-Fasciclin II, RRID:AB _ 528235 ), Anti-HA 1:1000, Cell Signaling Technology cat# 3724, RRID: B _ 1549585 ), anti-H3 (1:20000, Active Motif Cat# 39763, RID:AB _ 2650522 ), anti-H3K4me3 (Cell Signaling Techology Cat# 9751, RRID:AB _ 2616028 ), Anti-FLAG (Sigmaldrich Cat# F1804, RRID:AB _ 262044 ), Anti-vGLUT2 (1:200; Millipore #mab5504) Anti-TUJ (1:500; Genescript #A01627), Anti-Cyclophilin B (Thermofisher #PA1-027A), anti-DAC (1:50; DSHB cat# mAbdac2-2; RRID:AB _ 528190 ). ..

    Selection:

    Article Title: KDM5-mediated transcriptional activation of ribosomal protein genes alters translation efficiency to regulate mitochondrial metabolism in neurons.
    Article Snippet: .. A tet-inducible expression ystem was introduced, and lentivirus particles prepared rom the plasmid vectors; pLV_TRET_hNgn2_UBC_Puro RRID:A ddgene_61474) and FUdeltaGW -rtT A ( RRID: ddgene_19780), followed by treatment with doxycycline nd selection with puromycin. ntibodies he following primary antibodies were used: Anti-Fas2 1:25, Developmental Studies Hybridoma Bank (DSHB) at# 1D4 anti-Fasciclin II, RRID:AB _ 528235 ), Anti-HA 1:1000, Cell Signaling Technology cat# 3724, RRID: B _ 1549585 ), anti-H3 (1:20000, Active Motif Cat# 39763, RID:AB _ 2650522 ), anti-H3K4me3 (Cell Signaling Techology Cat# 9751, RRID:AB _ 2616028 ), Anti-FLAG (Sigmaldrich Cat# F1804, RRID:AB _ 262044 ), Anti-vGLUT2 (1:200; Millipore #mab5504) Anti-TUJ (1:500; Genescript #A01627), Anti-Cyclophilin B (Thermofisher #PA1-027A), anti-DAC (1:50; DSHB cat# mAbdac2-2; RRID:AB _ 528190 ). ..

    other:

    Article Title: A memory transcriptome time course reveals essential long-term memory transcription factors
    Article Snippet: To determine neurodevelopmental defects in the MB after knocking-down positive hits, we used anti-Fasciclin II (that identifies ‘β’ and γlobes) and mouse anti-Trio (1/10; DSHB AB_528494) (that marks α‘β’ and γ lobes).

    Article Title: KDM5-mediated transcriptional activation of ribosomal protein genes alters translation efficiency to regulate mitochondrial metabolism in neurons
    Article Snippet: The following primary antibodies were used: Anti-Fas2 (1:25, Developmental Studies Hybridoma Bank (DSHB) cat# 1D4 anti-Fasciclin II, RRID:AB_528235 ), Anti-HA (1:1000, Cell Signaling Technology cat# 3724, RRID:AB_1549585 ), anti-H3 (1:20000, Active Motif Cat# 39763, RRID:AB_2650522 ), anti-H3K4me3 (Cell Signaling Technology Cat# 9751, RRID:AB_2616028 ), Anti-FLAG (Sigma-Aldrich Cat# F1804, RRID:AB_262044 ), Anti-vGLUT2 (1:200; Millipore #mab5504) Anti-TUJ (1:500; Genescript #A01627), Anti-Cyclophilin B (Thermofisher #PA1-027A), anti-DAC (1:50; DSHB cat# mAbdac2-2; RRID:AB_528190 ).

    Immunohistochemistry:

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity.
    Article Snippet: .. Brains were dissected in PBS and fixed with ice cold 4% paraformaldehyde for 30 to 45 min. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary anti-bodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H1900) before imagining.

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic identity and translation capacity
    Article Snippet: .. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary anti-bodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H-1900) before imagining.

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity
    Article Snippet: .. Brains were dissected in PBS and fixed with ice cold 4% paraformaldehyde for 30 to 45 min. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary antibodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H-1900) before imagining.

    Next-Generation Sequencing:

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity.
    Article Snippet: .. Brains were dissected in PBS and fixed with ice cold 4% paraformaldehyde for 30 to 45 min. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary anti-bodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H1900) before imagining.

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic identity and translation capacity
    Article Snippet: .. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary anti-bodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H-1900) before imagining.

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity
    Article Snippet: .. Brains were dissected in PBS and fixed with ice cold 4% paraformaldehyde for 30 to 45 min. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary antibodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H-1900) before imagining.

    Incubation:

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity.
    Article Snippet: .. Brains were dissected in PBS and fixed with ice cold 4% paraformaldehyde for 30 to 45 min. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary anti-bodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H1900) before imagining.

    Article Title: Ataxia-associated DNA repair genes protect the Drosophila mushroom body and locomotor function against glutamate signaling-associated damage
    Article Snippet: .. Brains were incubated with the primary antibodies anti-FasII (DSHB, 1:5) or anti-dac (mAbdac1-1, DSHB, 1:125) in combination with anti-Asp175 (Cell signaling, 1:200) or anti-γH2AvD pS137 (Rockland, 1:200) for 2 d at 4°C. .. Brains were washed 5 times for 10 min in PBS-T and incubated for 2 h with secondary antibodies Alexa-568 red mouse (Life Technologies, 1:500) and Alexa-488 green rabbit (Invitrogen, 1:500) at room temperature.

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic identity and translation capacity
    Article Snippet: .. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary anti-bodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H-1900) before imagining.

    Article Title: Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity
    Article Snippet: .. Brains were dissected in PBS and fixed with ice cold 4% paraformaldehyde for 30 to 45 min. For immunohistochemistry, fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25, Developmental Studies Hybridoma Bank (DSHB): #1D4], or anti-dac (1:100, DSHB: mAbdac2-3), and secondary antibodies AlexaFluor 488 or 594 (1:300, Invitrogen: A1108 & A1105). .. Brains were then mounted in SlowFade Antifade (Invitrogen: S36972) or VectaShield (VectorLabs: H-1900) before imagining.



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